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frozen human peripheral blood mononuclear cells (pbmcs)  (STEMCELL Technologies Inc)

 
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    Structured Review

    STEMCELL Technologies Inc frozen human peripheral blood mononuclear cells (pbmcs)
    Frozen Human Peripheral Blood Mononuclear Cells (Pbmcs), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/frozen+human+peripheral+blood+mononuclear+cells+(pbmcs)/human+peripheral+blood+mononuclear+cells/us12325757-457-3-11
    Average 90 stars, based on 1 article reviews
    frozen human peripheral blood mononuclear cells (pbmcs) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Isolation:

    Article Title: Tri-specific binding molecules
    Article Snippet: Frozen human peripheral blood mononuclear cells (PBMCs) were obtained from Stemcell Technologies.

    Article Title: Cell trajectory modulation: rapid microfluidic biophysical profiling of CAR T cell functional phenotypes.
    Article Snippet: Frozen human peripheral blood mononuclear cells (PBMC) from 3 healthy donors were purchased from STEMCELL Technologies.

    Article Title: XMT-2056, a HER2-Directed STING Agonist Antibody-Drug Conjugate, Induces Innate Anti-Tumor Immune Responses by Acting on Cancer Cells and Tumor-Resident Immune Cells
    Article Snippet: 1 1 2 3 4 5 6 7 8

    Article Title: Biological sex and age influence GS-9620 activity ex vivo.
    Article Snippet: 273 274 275 PBMCs 276 Buffy coats were obtained from HIV-negative donors aged 17 years and older from the Gulf Coast 277 Regional Blood Center or frozen peripheral blood cells (PBMCs) from STEMCELL Technologies 278 were used (cat#70025).

    Article Title: AWT020: a novel fusion protein harnessing PD-1 blockade and selective IL-2 Cis-activation for enhanced anti-tumor immunity and diminished toxicity
    Article Snippet: Frozen human peripheral blood mononuclear cells (PBMCs) from healthy donors were obtained from STEMCELL Technologies.

    Article Title: Protease cleavable bispecific antibodies and uses thereof
    Article Snippet: Meanwhile, 2.5×107 frozen human peripheral blood mononuclear cells (PBMCs) purchased from STEMCELL Technologies were revived and cultured in 10 mL RPMI 1640 complete medium containing 50 U/mL IL-2 (Sigma) overnight.

    Article Title: Biological sex and age influence GS-9620 activity ex vivo
    Article Snippet: Buffy coats were obtained from HIV-negative donors aged 17 years and older from the Gulf Coast Regional Blood Center, or frozen PBMCs from STEMCELL Technologies were used (catalog 70025).

    Gradient Centrifugation:

    Article Title: Tri-specific binding molecules
    Article Snippet: Frozen human peripheral blood mononuclear cells (PBMCs) were obtained from Stemcell Technologies.

    Article Title: Cell trajectory modulation: rapid microfluidic biophysical profiling of CAR T cell functional phenotypes.
    Article Snippet: Frozen human peripheral blood mononuclear cells (PBMC) from 3 healthy donors were purchased from STEMCELL Technologies.

    Article Title: XMT-2056, a HER2-Directed STING Agonist Antibody-Drug Conjugate, Induces Innate Anti-Tumor Immune Responses by Acting on Cancer Cells and Tumor-Resident Immune Cells
    Article Snippet: 1 1 2 3 4 5 6 7 8

    Article Title: Biological sex and age influence GS-9620 activity ex vivo.
    Article Snippet: 273 274 275 PBMCs 276 Buffy coats were obtained from HIV-negative donors aged 17 years and older from the Gulf Coast 277 Regional Blood Center or frozen peripheral blood cells (PBMCs) from STEMCELL Technologies 278 were used (cat#70025).

    Article Title: AWT020: a novel fusion protein harnessing PD-1 blockade and selective IL-2 Cis-activation for enhanced anti-tumor immunity and diminished toxicity
    Article Snippet: Frozen human peripheral blood mononuclear cells (PBMCs) from healthy donors were obtained from STEMCELL Technologies.

    Article Title: Protease cleavable bispecific antibodies and uses thereof
    Article Snippet: Meanwhile, 2.5×107 frozen human peripheral blood mononuclear cells (PBMCs) purchased from STEMCELL Technologies were revived and cultured in 10 mL RPMI 1640 complete medium containing 50 U/mL IL-2 (Sigma) overnight.

    Article Title: Biological sex and age influence GS-9620 activity ex vivo
    Article Snippet: Buffy coats were obtained from HIV-negative donors aged 17 years and older from the Gulf Coast Regional Blood Center, or frozen PBMCs from STEMCELL Technologies were used (catalog 70025).



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    Image Search Results


    a diABZI-i inhibits cGAMP-induced IFNβ in PBMCs ( n = 3 | 1 donor) and diABZI-a1 induces IFNβ in PBMCs ( n = 3 | 3 donors, representative donor displayed). Data represented as mean with SD in graph. Source data are provided as a Source Data file. b Superposition of crystal structures (side view) of STING bound to diABZI-a1 (gray) and diABZI-i (purple). c diABZI-a1 and diABZI-i superposition over the STING binding pocket. d Cartoon depicting apical wing distance and beta sheet lid between STING monomers. e Superposition of crystal structures (top view) of STING bound to diABZI-a1 (gray) and diABZI-i (purple). f THIQi inhibits cGAMP-induced IFNβ in PBMCs ( n = 3 | 1 donor). Data represented as mean with SD in graph. Source data are provided as a Source Data file. g Crystal structure overlay (top view) of STING bound to THIQi (orange) and diABZI-i (purple). h Apical wing distance measurements from structures in this study and reference structures collected from the PDB.

    Journal: Nature Communications

    Article Title: Orthosteric STING inhibition elucidates molecular correction of SAVI STING

    doi: 10.1038/s41467-025-60632-5

    Figure Lengend Snippet: a diABZI-i inhibits cGAMP-induced IFNβ in PBMCs ( n = 3 | 1 donor) and diABZI-a1 induces IFNβ in PBMCs ( n = 3 | 3 donors, representative donor displayed). Data represented as mean with SD in graph. Source data are provided as a Source Data file. b Superposition of crystal structures (side view) of STING bound to diABZI-a1 (gray) and diABZI-i (purple). c diABZI-a1 and diABZI-i superposition over the STING binding pocket. d Cartoon depicting apical wing distance and beta sheet lid between STING monomers. e Superposition of crystal structures (top view) of STING bound to diABZI-a1 (gray) and diABZI-i (purple). f THIQi inhibits cGAMP-induced IFNβ in PBMCs ( n = 3 | 1 donor). Data represented as mean with SD in graph. Source data are provided as a Source Data file. g Crystal structure overlay (top view) of STING bound to THIQi (orange) and diABZI-i (purple). h Apical wing distance measurements from structures in this study and reference structures collected from the PDB.

    Article Snippet: Frozen human peripheral blood mononuclear cells (PBMCs, BioIVT) are gently thawed, suspended in complete medium [AIMV, 10% heat-inactivated FBS, 1% antibiotic-antimycotic, 1% sodium pyruvate, 1% MEM non-essential amino acids; ThermoFisher] and concentrated by low-speed centrifugation (400 × g, ambient temperature, five minutes).

    Techniques: Binding Assay